Identification of secreted factors in dental pulp cell-conditioned medium optimized for neuronal growth

With their potent regenerative and protective capacities, stem cell-derived conditioned media emerged as an effective alternative to cell therapy, have a prospect be manufactured pharmaceutical products for tissue regeneration applications. Our study investigates the neuroregenerative potential of human dental pulp cells (DPCs) medium (CM) defines optimization strategy DPC-CM enhanced neuronal outgrowth. Primary sensory neurons from mouse dorsal root ganglia were cultured with or without DPC-CM, lengths ?III-tubulin positive neurites measured. The impacts several manufacturing features duration conditioning, CM storage, preconditioning DPCs some factors on functional activity assessed neurite length. We observed that significantly outgrowth in concentration-dependent manner. frozen storage had no impact experimental outcomes 48 h DPC conditioning is optimal CM. To further understand feature we studied secretome by growth factor antibody array analysis revealed presence involved either neurogenesis, neuroprotection, angiogenesis, osteogenesis. B-27 supplement effect changing its composition factors. Here, show stimulate primary neurons. Moreover, identified secreted protein candidates can potentially promote this promising DPC-CM.